eCite Digital Repository

Histopathology in Pacific oyster (Crassostrea gigas) spat caused by the dinoflagellate Prorocentrum rhathymum


Pearce, I and Handlinger, JH and Hallegraeff, GM, Histopathology in Pacific oyster (Crassostrea gigas) spat caused by the dinoflagellate Prorocentrum rhathymum, Harmful Algae, 4, (1) pp. 61-74. ISSN 1568-9883 (2005) [Refereed Article]

DOI: doi:10.1016/j.hal.2003.11.002


The recognition of an apparent association between seasonal oyster spat mortalities (up to 40%) and high Prorocentrum rhathymum density in the Little Swanport Estuary, Tasmania, prompted further experimental investigation into the toxicity by this dinoflagellate. Standard brine shrimp, haemolysis assays and intraperitoneal mouse bioassays revealed fast acting toxins in methanol but not aqueous extracts of P. rhathymum, with mice dying in less than 20 min. Oyster bioassays involved feeding spat (4 mm shell width) for 21 consecutive days on a diet of cultured P. rhathymum at simulated bloom densities (10 4 cells ml -1). No oyster mortality was observed, however, histopathological signs of thin, dilated gut tubules and sloughing of gut cells resembled those seen in affected field samples. In contrast to field samples, gill pathology was also observed in experimental exposure oysters. © 2003 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Phycology (incl. marine grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Pearce, I (Dr Imojen Pearce)
UTAS Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:35132
Year Published:2005
Web of Science® Times Cited:52
Deposited By:Plant Science
Deposited On:2005-08-01
Last Modified:2022-11-04

Repository Staff Only: item control page