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Identification of a genetic locus modulating splenomegaly induced by granulocyte colony-stimulating factor in mice
journal contribution
posted on 2023-05-16, 16:48 authored by Roberts, AW, Hasegawa, M, Metcalf, D, Simon James FooteSimon James FooteClinically detectable splenomegaly and splenic rupture are uncommon but potentially life-threatening consequences of G-CSF administration. Increased spleen size in mice injected with G-CSF is a complex genetic trait amenable to investigation in experimental inter-strain crosses by quantitative trait analysis. A quantitative trait locus (QTL) with highly significant linkage (LCD 7.9) for splenomegaly was identified within a 22 centimorgan (cM) region on chromosome 1. Inheritance of a C57BL/6 haplotype in this region was associated with a greater spleen weight. The relevance of this locus was confirmed by analysing the responses of mice congenic for the distal 12 cM of this region (C57BL/6 and C57BL/6.SJL-Ptprc a Pep3 b ). Consistent with the QTL effect, mice lacking C57BL/6 alleles in this region had reduced splenomegaly induced by G-CSF. Intriguingly, peripheral blood neutrophilia and progenitor cell mobilisation responses to G-CSF were also significantly influenced.
History
Publication title
LeukemiaVolume
14Issue
4Pagination
657-661ISSN
0887-6924Department/School
Menzies Institute for Medical ResearchPublisher
Macmillan Publishers LtdPlace of publication
UKRepository Status
- Restricted