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Improvements in allelic discrimination of microsatellite markers using denaturing polyacrylamide gel electrophoresis

Citation

Symons, RCA and Marshall, VM and Foote, SJ, Improvements in allelic discrimination of microsatellite markers using denaturing polyacrylamide gel electrophoresis, Mammalian Genome, 11, (8) pp. 671-674. ISSN 0938-8990 (2000) [Refereed Article]

DOI: doi:10.1007/s003350010122

Abstract

Poor resolution, retarded progress of DNA through gels, and variable sizing of DNA fragments between and within gels hinder accurate genotyping of some simple sequence length polymorphism (SSLP) markers with the Perkin Elmer Applied Biosystems 377 Sequenator. These problems are similar to renaturation related problems observed in DNA sequencing gels. PCR products especially susceptible to these problems are shown to have higher melting temperatures (T(m)) than others. Gels containing increased concentrations of denaturants allow greater accuracy in allelic discrimination. This is especially beneficial where quantification is necessary.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Medical Genetics (excl. Cancer Genetics)
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Foote, SJ (Professor Simon Foote)
ID Code:35116
Year Published:2000
Deposited By:Menzies Centre
Deposited On:2005-08-01
Last Modified:2011-11-18
Downloads:0

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