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Improvements in allelic discrimination of microsatellite markers using denaturing polyacrylamide gel electrophoresis
Citation
Symons, RCA and Marshall, VM and Foote, SJ, Improvements in allelic discrimination of microsatellite markers using denaturing polyacrylamide gel electrophoresis, Mammalian Genome, 11, (8) pp. 671-674. ISSN 0938-8990 (2000) [Refereed Article]
DOI: doi:10.1007/s003350010122
Abstract
Poor resolution, retarded progress of DNA through gels, and variable sizing of DNA fragments between and within gels hinder accurate genotyping of some simple sequence length polymorphism (SSLP) markers with the Perkin Elmer Applied Biosystems 377 Sequenator. These problems are similar to renaturation related problems observed in DNA sequencing gels. PCR products especially susceptible to these problems are shown to have higher melting temperatures (T(m)) than others. Gels containing increased concentrations of denaturants allow greater accuracy in allelic discrimination. This is especially beneficial where quantification is necessary.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Medical and Health Sciences |
| Research Group: | Clinical Sciences |
| Research Field: | Medical Genetics (excl. Cancer Genetics) |
| Objective Division: | Health |
| Objective Group: | Other Health |
| Objective Field: | Health not elsewhere classified |
| Author: | Foote, SJ (Professor Simon Foote) |
| ID Code: | 35116 |
| Year Published: | 2000 |
| Deposited By: | Menzies Centre |
| Deposited On: | 2005-08-01 |
| Last Modified: | 2011-11-18 |
| Downloads: | 0 |
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