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Biogeographic and quantitative analyses of abundant uncultivated gamma-proteobacterial clades from marine sediment

Citation

Bowman, JP and McCammon, SA and Dann, AL, Biogeographic and quantitative analyses of abundant uncultivated gamma-proteobacterial clades from marine sediment , Microbial Ecology, 49, (3) pp. 451-460. ISSN 0095-3628 (2005) [Refereed Article]

DOI: doi:10.1007/s00248-004-0070-2

Abstract

16S rRNA gene-based molecular analyses revealed the presence of several large and so far uncultivated clades within class γ-Proteobacteria, designated γ-proteobacterial marine sediment (GMS) clades 1 to 4, in marine sediment. The GMS clades appear only indigenous to marine sediment and so far have an unknown functionality. SYBR Green-based real-time PCR analyses using GMS clade-specific primers indicated GMS clades were a significant part of the bacterial community (0.3-8.7% of total 16S rRNA genes) in both polar and temperate marine sediment samples. Univariate statistical analyses indicated that GMS clade communities were indistinguishable in two temperate coastal sediment samples even though these possessed very different mean grain sizes, organic contents, and organic loading rates. GMS clade communities were slightly different (p < 0.05) between polar and temperate sites, suggesting that psychrophilic adaptation among GMS clade taxa corresponds only to subtle phylogenetic differences. Similar levels of difference were also observed through a sediment core reflecting that through the sediment core history, which spanned ∼3000 years, GMS clonal diversity shifted only marginally. © Springer Science+Business Media, Inc. 2005.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Other Environment
Objective Field:Environment not elsewhere classified
Author:Bowman, JP (Associate Professor John Bowman)
Author:McCammon, SA (Ms Sharee McCammon)
Author:Dann, AL (Dr Alison Dann)
ID Code:35089
Year Published:2005
Web of Science® Times Cited:22
Deposited By:Agricultural Science
Deposited On:2005-08-01
Last Modified:2007-11-09
Downloads:0

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