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Growth limits of Listeria monocytogenes as a function of temperature, pH, NaCl, and lactic acid


Tienungoon, S and Ratkowsky, DA and McMeekin, TA and Ross, T, Growth limits of Listeria monocytogenes as a function of temperature, pH, NaCl, and lactic acid, Applied and Environmental Microbiology, 66, (11) pp. 4979-4987. ISSN 0099-2240 (2000) [Refereed Article]

DOI: doi:10.1128/AEM.66.11.4979-4987.2000


Models describing the limits of growth of pathogens under multiple constraints will aid management of the safety of foods which are sporadically contaminated with pathogens and for which subsequent growth of the pathogen would significantly increase the risk of food-borne illness. We modeled the effects of temperature, water activity, pH, and lactic acid levels on the growth of two strains of Listeria monocytogenes in tryptone soya yeast extract broth. The results could be divided unambiguously into 'growth is possible' or 'growth is not possible' classes. We observed minor differences in growth characteristics of the two L. monocytogenes strains. The data follow a binomial probability distribution and may be modeled using logistic regression. The model used is derived from a growth rate model in a manner similar to that described in a previously published work (K. A. Presser, T. Ross, and D. A. Ratkowsky, Appl. Environ. Microbiol. 64:1773-1779, 1998). We used 'nonlinear logistic regression' to estimate the model parameters and developed a relatively simple model that describes our experimental data well. The fitted equations also described well the growth limits of all strains of L. monocytogenes reported in the literature, except at temperatures beyond the limits of the experimental data used to develop the model (3 to 35°C). The models developed will improve the rigor of microbial food safety risk assessment and provide quantitative data in a concise form for the development of safer food products and processes.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Food safety
UTAS Author:Tienungoon, S (Ms Suwunna Tienungoon)
UTAS Author:Ratkowsky, DA (Dr David Ratkowsky)
UTAS Author:McMeekin, TA (Professor Thomas McMeekin)
UTAS Author:Ross, T (Professor Tom Ross)
ID Code:34089
Year Published:2000
Web of Science® Times Cited:182
Deposited By:Agricultural Science
Deposited On:2005-07-25
Last Modified:2005-07-25

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