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GM-CSF promoter chromatin remodelling and gene transcription display distinct signal and transcription factor requirements

Citation

Brettingham-Moore, KH and Rao, S and Jeulich, T and Shannon, MF and Holloway, AF, GM-CSF promoter chromatin remodelling and gene transcription display distinct signal and transcription factor requirements, Nucleic Acids Research, 33, (1) pp. 225-234. ISSN 0305-1048 (2005) [Refereed Article]

DOI: doi:10.1093/nar/gki161

Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) plays a key role in myeloid cell function and is rapidly and transiently expressed in T cells in response to immune or inflammatory stimuli. Induction of GM-CSF gene expression is accompanied by changes in chromatin structure across the proximal promoter region of the gene. We show that the promoter remodelling and subsequent gene transcription occurs with distinct signal and transcription factor requirements. Activation of the protein kinase C (PKC) signalling pathway is sufficient to induce changes in chromatin structure across the promoter, but both the PKC and calcium signalling pathways are required forefficient gene transcription. Although NFAT transcription factors contribute to GM-CSF gene transcription, they are not required for promoter remodelling. However, the presence of the nuclear factor-κB transcription factor, c-Rel, in the nucleus is strongly correlated with and required for the events of chromatin remodelling. © Oxford University Press 2005; all rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Biochemistry and Cell Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Brettingham-Moore, KH (Dr Kate Brettingham-Moore)
Author:Holloway, AF (Associate Professor Adele Holloway)
ID Code:34043
Year Published:2005
Web of Science® Times Cited:25
Deposited By:Pathology
Deposited On:2005-08-01
Last Modified:2012-04-20
Downloads:0

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