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Screening plants for salt tolerance by measuring K+ flux: a case study for barley

Citation

Chen, Z and Newman, IA and Zhou, M and Mendham, NJ and Zhang, G and Shabala, SN, Screening plants for salt tolerance by measuring K+ flux: a case study for barley, Plant, Cell and Environment , 28, (10) pp. 1230-1246. ISSN 0140-7791 (2005) [Refereed Article]

DOI: doi:10.1111/j.1365-3040.2005.01364.x

Abstract

Development of salt-tolerant genotypes is central both to remediation of salinity-affected land and to meet increasing global food demand, which has been driving expansion of cropping into marginal areas. The bottleneck of any breeding programme is the lack of a reliable screening technique. This study tested the hypothesis that the ability of plants to retain K + under saline conditions is central to their salt tolerance. Using seven barley cultivars contrasting in salt tolerance (CM72, Numar, ZUG293, ZUG95, Franklin, Gairdner, ZUG403), a comprehensive study was undertaken of whole-plant (growth rate, biomass, net CO 2 assimilation, chlorophyll fluorescence, root and leaf elemental and water content) and cellular (net fluxes of H +, K +, Na + and Ca 2+) responses to various concentrations of NaCl (20-320 mM). Na + selective microelectrodes were found to be unsuitable for screening purposes because of non-ideal selectivity of the commercially available Na + LIX. At the same time, our results show very strong negative correlation between the magnitude of K + efflux from the root and salt tolerance of a particular cultivar. K + efflux from the mature root zone of intact 3-day-old seedlings following 40 min pretreatment with 80 mM NaCl was found to be a reliable screening indicator for salinity tolerance in barley. As a faster and more cost-effective alternative to microelectrode measurements, a procedure was developed enabling rapid screening of large numbers of seedlings, based on amount of K + leaked from plant roots after exposure to NaCl. © 2005 Blackwell Publishing Ltd.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Chen, Z (Mr Zhonghua Chen)
Author:Newman, IA (Dr Ian Newman)
Author:Zhou, M (Professor Meixue Zhou)
Author:Mendham, NJ (Dr Neville Mendham)
Author:Shabala, SN (Professor Sergey Shabala)
ID Code:33724
Year Published:2005
Web of Science® Times Cited:221
Deposited By:Agricultural Science
Deposited On:2005-08-01
Last Modified:2011-12-06
Downloads:0

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