eCite Digital Repository
Screening plants for salt tolerance by measuring K+ flux: a case study for barley
Citation
Chen, Z and Newman, IA and Zhou, M and Mendham, NJ and Zhang, G and Shabala, SN, Screening plants for salt tolerance by measuring K+ flux: a case study for barley, Plant, Cell and Environment , 28, (10) pp. 1230-1246. ISSN 0140-7791 (2005) [Refereed Article]
DOI: doi:10.1111/j.1365-3040.2005.01364.x
Abstract
Development of salt-tolerant genotypes is central both to remediation of salinity-affected land and to meet increasing global food demand, which has been driving expansion of cropping into marginal areas. The bottleneck of any breeding programme is the lack of a reliable screening technique. This study tested the hypothesis that the ability of plants to retain K + under saline conditions is central to their salt tolerance. Using seven barley cultivars contrasting in salt tolerance (CM72, Numar, ZUG293, ZUG95, Franklin, Gairdner, ZUG403), a comprehensive study was undertaken of whole-plant (growth rate, biomass, net CO 2 assimilation, chlorophyll fluorescence, root and leaf elemental and water content) and cellular (net fluxes of H +, K +, Na + and Ca 2+) responses to various concentrations of NaCl (20-320 mM). Na + selective microelectrodes were found to be unsuitable for screening purposes because of non-ideal selectivity of the commercially available Na + LIX. At the same time, our results show very strong negative correlation between the magnitude of K + efflux from the root and salt tolerance of a particular cultivar. K + efflux from the mature root zone of intact 3-day-old seedlings following 40 min pretreatment with 80 mM NaCl was found to be a reliable screening indicator for salinity tolerance in barley. As a faster and more cost-effective alternative to microelectrode measurements, a procedure was developed enabling rapid screening of large numbers of seedlings, based on amount of K + leaked from plant roots after exposure to NaCl. © 2005 Blackwell Publishing Ltd.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Crop and pasture production |
| Research Field: | Crop and pasture biochemistry and physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Barley |
| UTAS Author: | Chen, Z (Mr Zhonghua Chen) |
| UTAS Author: | Newman, IA (Dr Ian Newman) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| UTAS Author: | Mendham, NJ (Dr Neville Mendham) |
| UTAS Author: | Shabala, SN (Professor Sergey Shabala) |
| ID Code: | 33724 |
| Year Published: | 2005 |
| Web of Science® Times Cited: | 343 |
| Deposited By: | Agricultural Science |
| Deposited On: | 2005-08-01 |
| Last Modified: | 2011-12-06 |
| Downloads: | 0 |
Repository Staff Only: item control page