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Microelectrode ion and O2 fluxes measurements reveal differential sensitivity of barley root tissues to hypoxia

Citation

Pang, J and Newman, IA and Mendham, NJ and Zhou, M and Shabala, SN, Microelectrode ion and O2 fluxes measurements reveal differential sensitivity of barley root tissues to hypoxia, Plant, Cell and Environment , 29, (6) pp. 1107-1121. ISSN 0140-7791 (2006) [Refereed Article]

DOI: doi:10.1111/j.1365-3040.2005.01486.x

Abstract

Hypoxia-induced changes in net H +, K + and O 2 fluxes across the plasma membrane (PM) of epidermal root cells were measured using the non-invasive microelectrode ion flux measurement (MIFE) system in elongation, meristem and mature root zones of two barley (Hordeum vulgare L.) varieties contrasting in their waterlogging (WL) tolerance. The ultimate goal of this study was to shed light on the mechanisms underlying effects of WL on plant nutrient acquisition and mechanisms of WL tolerance in barley. Our measurements revealed that functionally different barley root zones have rather different O 2 requirements, with the highest O 2 influx being in the elongation zone of the root at about 1 mm from the tip. Oxygen deprivation has qualitatively different effects on the activity of PM ion transporters in mature and elongation zones. In the mature zone, hypoxic treatment caused a very sharp decline in K + uptake in the WL sensitive variety Naso Nijo, but did not reduce K + influx in the WL tolerant TX9425 variety. In the elongation zone, onset of hypoxia enhanced K + uptake from roots of both cultivars. Pharmacological experiments suggested that hypoxia-induced K + flux responses are likely to be mediated by both K + -inward- (KIR) and non-selective cation channels (NSCC) in the elongation zone, while in the mature zone K + -outward- (KOR) channels are the key contributors. Overall, our results suggest that oxygen deprivation has an immediate and substantial effect on root ion flux patterns, and that this effect is different in WL-sensitive and WL-tolerant cultivars. To what extent this difference in ion flux response to hypoxia is a factor conferring WL tolerance in barley remains to be answered in future studies. © 2006 Blackwell Publishing Ltd.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Pang, J (Ms Jiayin Pang)
Author:Newman, IA (Dr Ian Newman)
Author:Mendham, NJ (Dr Neville Mendham)
Author:Zhou, M (Professor Meixue Zhou)
Author:Shabala, SN (Professor Sergey Shabala)
ID Code:33693
Year Published:2006
Web of Science® Times Cited:45
Deposited By:Agricultural Science
Deposited On:2006-08-01
Last Modified:2007-04-17
Downloads:0

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