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Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae


Tristram, SG and Hawes, RA and Souprounov, J, Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae, Journal of Antimicrobial Chemotherapy, 56, (3) pp. 481-484 . ISSN 0305-7453 (2005) [Refereed Article]

DOI: doi:10.1093/jac/dki238


Objectives: To determine if the TEM β-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters. Methods: Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the blaTEM genes of a collection of TEM-positive strains. Results: All blaTEM genes were found to be blaTEM-1 or derivatives thereof and none blaTEM-2 The blaTEM genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution. Conclusions: The genetic features of blaTEM genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Tristram, SG (Dr Stephen Tristram)
UTAS Author:Hawes, RA (Ms Rebecca Hawes)
UTAS Author:Souprounov, J (Ms Juliana Souprounov)
ID Code:33543
Year Published:2005
Web of Science® Times Cited:15
Deposited By:Health Sciences A
Deposited On:2005-08-01
Last Modified:2011-10-12

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