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Diagnostic polymerase chain reaction assay to detect Kudoa neurophila (Myxozoa:Multivalvulida) in a marine finfish hatchery

Citation

Grossel, GW and Handlinger, JH and Battaglene, SC and Munday, BL, Diagnostic polymerase chain reaction assay to detect Kudoa neurophila (Myxozoa:Multivalvulida) in a marine finfish hatchery, Diseases of Aquatic Organisms, 64, (2) pp. 141-149. ISSN 0177-5103 (2005) [Refereed Article]

DOI: doi:10.3354/dao064141

Abstract

A single-round polymerase chain reaction (PCR) diagnostic assay was developed from a small subunit ribosomal DNA (SSU rDNA) gene sequence to detect the myxozoan parasite Kudoa neurophila, the causative agent of myxozoan disease in the hatchery reared marine finfish, striped trumpeter Latris lineata (Forster). The assay was developed for use as a disease control management tool in a hatchery system specifically designed to research and produce marine finfish such as striped trumpeter juveniles for aquaculture. The assay is sufficiently species specific and sensitive enough to detect a small fragment of the parasite's SSU rDNA. At the lower limits of detection, the test is consistently positive to an estimated 0.1 spore or 60 fg of parasite DNA per 25 μl PCR reaction in serial dilution and positive to an estimated 0.1 spore in 25 mg of infected fish CNS tissue (4 spores g-1). Specifically, the test is capable of detecting early stages of the life cycle within the fish host and consequently diagnosing an infection not normally detected using traditional histological techniques. The test is also effective for screening water supplies and prey species cultures throughout the hatchery system to determine bio-security efficacy, to assist in identification of an alternate or other primary fish host, to indicate the location of potential disease reservoirs, and to enable a targeted approach to disease prevention. © Inter-Research 2005.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Grossel, GW (Mr Geoffrey Grossel)
Author:Handlinger, JH (Dr Judith Handlinger)
Author:Battaglene, SC (Associate Professor Stephen Battaglene)
Author:Munday, BL (Dr Barry Munday)
ID Code:33506
Year Published:2005
Web of Science® Times Cited:10
Deposited By:Health Sciences A
Deposited On:2005-08-01
Last Modified:2006-04-12
Downloads:0

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