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Effect of Phorbol Esters on the Distribution and Total Activity of Protein Kinase C in the Perfused Rat Heart

Citation

Rattigan, S and Davison, AG and Cleland, PJ and Clark, MG, Effect of Phorbol Esters on the Distribution and Total Activity of Protein Kinase C in the Perfused Rat Heart, International Journal of Biochemistry, 21, (12) pp. 1415-1420. ISSN 0020-711X (1989) [Refereed Article]

DOI: doi:10.1016/0020-711X(89)90164-X

Abstract

1. 1. The perfused rat heart was treated with the tumour-promoter and protein kinase C activator, phorbol 12-myristate 13-acetate and the distribution of protein kinase C activity between cytosolic and particulate fractions determined. 2. 2. Phorbol ester treatment led to a rapid loss of protein kinase C activity from the cytosol (t0.5 = 2 min) with a corresponding translocation into the particulate fraction. Translocated protein kinase C activity was tightly bound to the particulate fraction, could only be extracted with buffers containing 2% Triton X-100 and could therefore be misinterpreted as being down-regulated. 3. 3. Claims of rapid down-regulation of protein kinase C activity by phorbol esters need to be supported by rigorous procedures for extraction of the partieulate material. © 1989.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Medicinal and Biomolecular Chemistry
Research Field:Medicinal and Biomolecular Chemistry not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Rattigan, S (Professor Stephen Rattigan)
Author:Davison, AG (Associate Professor Aidan Davison)
Author:Cleland, PJ (Mr Perry Jason Cleland)
Author:Clark, MG (Professor Michael Clark)
ID Code:33253
Year Published:1989
Web of Science® Times Cited:6
Deposited By:Geography and Environmental Studies
Deposited On:2005-07-06
Last Modified:2005-07-06
Downloads:0

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