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Probing for pH-Regulated Genes in Sinorhizobium medicae Using Transcriptional Analysis


Tiwari, RP and Reeve, WG and Fenner, BJ and Dilworth, MJ and Glenn, AR and Howieson, JG, Probing for pH-Regulated Genes in Sinorhizobium medicae Using Transcriptional Analysis, Journal of Molecular Microbiology and Biotechnology, 7, (3) pp. 133-139. ISSN 1464-1801 (2004) [Refereed Article]

DOI: doi:10.1159/000078656


The low pH sensitivity of Sinorhizobium species is one of the major causes of reduced productivity of Medicago species (such as lucerne) sown in acidic soils. To investigate the pH response of an acid-tolerant Sinorhizobium medicae strain, a pool of random promoter fusions to gusA was created using minitransposon insertional mutagenesis. Acid-activated expression was identified in 11 mutants; rhizobial DNA flanking insertions in 10 mutants could be cloned and the DNA sequences obtained were used to interrogate the genome database of Sinorhizobium meliloti strain 1021. Acid activated expression was detected for fixNO, kdpC, lpiA, and phrR and for genes encoding a putative lipoprotein, two ABC-transporter components, a putative DNA ligase and a MPA1-family protein. These findings implicate cytochrome synthesis, potassium ion cycling, lipid biosynthesis and transport processes as key components of pH response in S. medicae. Copyright © 2004 S. Karger AG, Basel.

Item Details

Item Type:Refereed Article
Research Division:Environmental Sciences
Research Group:Soil sciences
Research Field:Soil sciences not elsewhere classified
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Glenn, AR (Professor Andrew Glenn)
ID Code:33138
Year Published:2004
Web of Science® Times Cited:14
Deposited By:Research Division
Deposited On:2004-08-01
Last Modified:2011-11-08

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