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Probing for pH-Regulated Proteins in Sinorhizobium medicae Using Proteomic Analysis

Citation

Reeve, WG and Tiwari, RP and Guerreiro, N and Stubbs, J and Dilworth, MJ and Glenn, AR and Rolfe, BG and Djordjevic, MA and Howieson, JG, Probing for pH-Regulated Proteins in Sinorhizobium medicae Using Proteomic Analysis, Journal of Molecular Microbiology and Biotechnology, 7, (3) pp. 140-147. ISSN 1464-1801 (2004) [Refereed Article]

DOI: doi:10.1159/000078657

Abstract

To elucidate the mechanisms of pH response in an acid-tolerant Sinorhizobium medicae strain we have identifi ed acid-activated gene transcription and now complement this approach by using a proteomic analysis to identify the changes that occur following exposure to acidity. Protein profi les of persistently or transiently acid-stressed S. medicae cells were compared to those grown in pH neutral, buffered media. Fifty pH-regulated proteins were identified; N-terminal sequences for 15 of these were obtained using the Edman degradation. Transient acid exposure downregulated GlnA and GlnK and upregulated a hypothetical protein. Continuing acid exposure downregulated ClpP, an ABC transporter, a hypothetical protein, a lipoprotein, the Trp-like repressor WrbA1 and upregulated DegP, fructose bisphosphate aldolase, GroES, malate dehydrogenase and two hypothetical proteins. These findings implicate proteolytic, chaperone and transport processes as key components of pH response in S. medicae . Copyright © 2004 S. Karger AG, Basel.

Item Details

Item Type:Refereed Article
Research Division:Environmental Sciences
Research Group:Soil Sciences
Research Field:Soil Sciences not elsewhere classified
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Horticultural Crops not elsewhere classified
Author:Glenn, AR (Professor Andrew Glenn)
ID Code:33137
Year Published:2004
Web of Science® Times Cited:13
Deposited By:Research Division
Deposited On:2004-08-01
Last Modified:2011-11-08
Downloads:0

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