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Excretory/secretory products of sheep abomasal nematode parasites cause vacuolation and increased neutral red uptake by HeLa cells

Citation

Przemeck, SMC and Huber, A and Brown, SC and Pedley, KC and Simpson, HV, Excretory/secretory products of sheep abomasal nematode parasites cause vacuolation and increased neutral red uptake by HeLa cells, Parasitology Research, 95, (3) pp. 213-217. ISSN 0932-0113 (2005) [Refereed Article]

DOI: doi:10.1007/s00436-004-1280-8

Abstract

Excretory/secretory (ES) products of Ostertagia (Teladorsagia) circumcincta and Haemonchus contortus have been implicated in the inhibition of gastric acid secretion and vacuolation, and the loss of parietal cells associated with abomasal parasitism. Vacuolation of epithelial (HeLa) cells caused by adult O. circumcincta or L3 O. circumcincta or H. contortus ES products have been examined by differential interference contrast microscopy and by the neutral red uptake assay. ES products caused visible vacuolation of HeLa cells, and this effect was enhanced by 8 mM NH4Cl. Some parasite ES products caused a marked detachment of cells from the coverslip. At lower concentrations of ES products, neutral red uptake was usually increased above the control, but at higher concentrations of ES products, uptake was often decreased, probably because of cell detachment. Although generally consistent with direct observations of HeLa cell vacuolation by parasite chemicals, neutral red uptake was not a satisfactory quantitative assay. © Springer-Verlag 2005.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Veterinary Sciences
Research Field:Veterinary Parasitology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock Raising
Objective Field:Sheep - Meat
Author:Brown, SC (Dr Simon Brown)
ID Code:33107
Year Published:2005
Web of Science® Times Cited:9
Deposited By:Health Sciences A
Deposited On:2005-08-01
Last Modified:2006-04-01
Downloads:0

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