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Capillary electrophoresis with on-chip four-electrode capacitively coupled conductivity detection for application in bioanalysis


Guijt, RM and Baltussen, E and van der Steen, G and Frank, H and Billiet, H and Schalkhammer, T and Laugere, F and Vellekoop, M and Berthold, A and Sarro, L and van Dedem, GWK, Capillary electrophoresis with on-chip four-electrode capacitively coupled conductivity detection for application in bioanalysis, Electrophoresis, 22, (12) pp. 2537-2541. ISSN 0173-0835 (2001) [Refereed Article]

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DOI: doi:10.1002/1522-2683(200107)22:12<2537::AID-ELPS2537>3.0.CO;2-C


Microchip capillary electrophoresis (CE) with integrated four-electrode capacitively coupled conductivity detection is presented. Conductivity detection is a universal detection technique that is relatively independent on the detection pathlength and, especially important for chip-based analysis, is compatible with miniaturization and on-chip integration. The glass microchip structure consists of a 6 cm etched channel (20 m70 m cross section) with silicon nitride covered walls. In the channel, a 30 nm thick silicon carbide layer covers the electrodes to enable capacitive coupling with the liquid inside the channel as well as to prevent interference of the applied separation field. The detector response was found to be linear over the concentration range from 20 M up to 2 mM. Detection limits were at the low M level. Separation of two short peptides with a pI of respectively 5.38 and 4.87 at the 1 mM level demonstrates the applicability for biochemical analysis. At a relatively low separation field strength (50 V/cm) plate numbers in the order of 3500 were achieved. Results obtained with the microdevice compared well with those obtained in a bench scale CE instrument using UV detection under similar conditions.

Item Details

Item Type:Refereed Article
Keywords:Capillary electrophoresis On-chip conductivity detection Peptides
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Instrumental methods (excl. immunological and bioassay methods)
Objective Division:Manufacturing
Objective Group:Instrumentation
Objective Field:Scientific instruments
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:32949
Year Published:2001
Web of Science® Times Cited:96
Deposited By:Chemistry
Deposited On:2005-06-06
Last Modified:2011-08-12

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