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On-Chip Contactless Four-Electrode Conductivity Detection for Capillary Electrophoresis Devices
Citation
Laugere, F and Guijt, RM and Bastemeijer, J and van der Steen, G and Berthold, A and Baltussen, E and Sarro, P and van Dedem, GWK and Vellekoop, M and Bossche, A, On-Chip Contactless Four-Electrode Conductivity Detection for Capillary Electrophoresis Devices, Analytical Chemistry, 75, (2) pp. 306-312. ISSN 0003-2700 (2003) [Refereed Article]
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Copyright Statement
© 2003 American Chemical Society
Abstract
In this contribution, a capillary electrophoresis microdevice with an integrated on-chip contactless four-electrode conductivity detector is presented. A 6-cm-long, 70-ìm-wide, and 20-ìm-deep channel was etched in a glass substrate that was bonded to a second glass substrate in order to form a sealed channel. Four contactless electrodes (metal electrodes covered by 30-nm silicon carbide) were deposited and patterned on the second glass substrate for on-chip conductivity detection. Contactless conductivity detection was performed in either a two- or a four-electrode configuration. Experimental results confirmed the improved characteristics of the four-electrode configuration over the classical two-electrode detection setup. The four-electrode configuration allows for sensitive detection for varying carrier−electrolyte background conductivity without the need for adjustment of the measurement frequency. Reproducible electrophoretic separations of three inorganic cations (K+, Na+, Li+) and six organic acids are presented. Detection as low as 5 ìM for potassium was demonstrated.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Instrumental Methods (excl. Immunological and Bioassay Methods) |
| Objective Division: | Manufacturing |
| Objective Group: | Instrumentation |
| Objective Field: | Scientific Instruments |
| Author: | Guijt, RM (Dr Rosanne Guijt) |
| ID Code: | 32947 |
| Year Published: | 2003 |
| Web of Science® Times Cited: | 77 |
| Deposited By: | Chemistry |
| Deposited On: | 2005-06-06 |
| Last Modified: | 2011-08-12 |
| Downloads: | 1 View Download Statistics |
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