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Microchip-Based Purification of DNA from Biological Samples

Citation

Breadmore, MC and Wolfe, KA and Arcibal, IG and Leung, WK and Dickson, D and Giordano, BC and Power, ME and Ferrance, JP and Feldman, SH and Norris, PM and Landers, JP, Microchip-Based Purification of DNA from Biological Samples, Analytical Chemistry, 75, (8) pp. 1880-1886. ISSN 0003-2700 (2003) [Refereed Article]


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Copyright Statement

Copyright 2003 American Chemical Society

Official URL: http://pubs.acs.org/

DOI: doi:10.1021/ac0204855

Abstract

A microchip solid-phase extraction method for purification of DNA from biological samples, such as blood, is demonstrated. Silica beads were packed into glass microchips and the beads immobilized with sol−gel to provide a stable and reproducible solid phase onto which DNA could be adsorbed. Optimization of the DNA loading conditions established a higher DNA recovery at pH 6.1 than 7.6. This lower pH also allowed for the flow rate to be increased, resulting in a decrease in extraction time from 25 min to less than 15 min. Using this procedure, template genomic DNA from human whole blood was purified on the microchip platform with the only sample preparation being mixing of the blood with load buffer prior to loading on the microchip device. Comparison between the microchip SPE (μchipSPE) procedure and a commercial microcentrifuge method showed comparable amounts of PCR-amplifiable DNA could be isolated from cultures of Salmonella typhimurium. The greatest potential of the μchipSPE device was illustrated by purifying DNA from spores from the vaccine strain of Bacillus anthracis, where eventual integration of SPE, PCR, and separation on a single microdevice could potentially enable complete detection of the infectious agent in less than 30 min.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:32938
Year Published:2003
Web of Science® Times Cited:269
Deposited By:Chemistry
Deposited On:2005-06-06
Last Modified:2010-03-01
Downloads:10 View Download Statistics

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