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Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon

Citation

Watts, M and Pankhurst, NW and King, HR and Geraghty, DP, Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon, Comparative Biochemistry and Physiology. Part A: Molecular & Integrated Physiology, 140, (3) pp. 377-383. ISSN 1095-6433 (2005) [Refereed Article]

DOI: doi:10.1016/j.cbpb.2005.02.002

Abstract

In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish held at 14, 18 or 22°C over the austral period of peak vitellogenesis (February to April). With one-site binding analysis, no temperature related difference in either receptor affinity (Kd) or number (B max) was found at each sampling point, but there was an apparent decrease in both affinity and number at each temperature over the period of the study. However, some analyses, notably at 22°C during February, were best described using a two-site binding model. At this temperature and time, there was a clear separation of binding affinity into high and low components (K d=0.67±S.E. 0.05 and 20±S.E. 5.6 nM, respectively) (n=4), which suggests that February was a critical time of temperature related hepatic sensitivity to estrogen. These results support those of other studies where we found that February was also a sensitive time with respect to temperature impairment of in vitro follicular estrogen synthesis, and the greatest period of in vivo temperature sensitivity. © 2005 Elsevier Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Watts, M (Dr Marianne Watts)
Author:King, HR (Mr Henry Robert King)
Author:Geraghty, DP (Professor Dominic Geraghty)
ID Code:32835
Year Published:2005
Web of Science® Times Cited:11
Deposited By:TAFI - Aquaculture
Deposited On:2005-08-01
Last Modified:2010-06-05
Downloads:0

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