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Plant Cell Growth and Ion Flux Responses to the Streptomycete Phytotoxin Thaxtomin A: Calcium and Hydrogen Flux Patterns Revealed by the Non-invasive MIFE Technique
Citation
Tegg, RS and Melian, L and Wilson, CR and Shabala, SN, Plant Cell Growth and Ion Flux Responses to the Streptomycete Phytotoxin Thaxtomin A: Calcium and Hydrogen Flux Patterns Revealed by the Non-invasive MIFE Technique, Plant and Cell Physiology, 46, (4) pp. 638-648. ISSN 0032-0781 (2005) [Refereed Article]
Abstract
Thaxtomin A, a key phytotoxin produced by plant pathogenic Streptomyces sp., is implicit in common scab disease expression in potato. Primary targets and modes of action of thaxtomin A toxicity in plant cells are not well understood. In this work, early signalling events associated with thaxtomin A toxicity were studied using the ion-selective microelectrode ion flux estimation (MIFE) technique. Thaxtomin A-induced changes in net ion fluxes were measured across the plasma membrane (PM) of root and pollen tube tissue in Arabidopsis thaliana and tomato. Within a minute after toxin application, a rapid and short-lived Ca2+ influx was observed. Well ahead of the marked inhibition of root growth, a significant shift towards net H+ efflux across the PM occurred in all tissues. Similar to root tissues, thaxtomin A significantly modified ion flux profiles from growing pollen tubes. Thaxtomin A was more effective in young, physiologically active tissues (root elongation zone or pollen tube apex), suggesting a higher density of thaxtomin A-binding sites in these regions. Overall, our data provide the first evidence that thaxtomin A triggers an early signalling cascade, which may be crucial in plant-pathogen interactions. It also suggests a possible interaction between thaxtomin A and PM auxin receptors, as revealed from experiments on the auxin-sensitive ucu2-2/gi2 A. thaliana mutant. © 2005 JSPP.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Horticultural crops |
Objective Field: | Field grown vegetable crops |
UTAS Author: | Tegg, RS (Dr Robert Tegg) |
UTAS Author: | Melian, L (Dr Lolita Melian) |
UTAS Author: | Wilson, CR (Professor Calum Wilson) |
UTAS Author: | Shabala, SN (Professor Sergey Shabala) |
ID Code: | 32785 |
Year Published: | 2005 |
Web of Science® Times Cited: | 52 |
Deposited By: | Agricultural Science |
Deposited On: | 2005-08-01 |
Last Modified: | 2006-05-03 |
Downloads: | 0 |
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