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Digestive capabilities reflect the major food sources in three species of talitrid amphipods


Johnston, MD and Johnston, DJ and Richardson, AMM, Digestive capabilities reflect the major food sources in three species of talitrid amphipods, Comparative Biochemistry and Physiology. Part B: Biochemistry and Molecular Biology, 140, (2) pp. 251-257. ISSN 1096-4959 (2005) [Refereed Article]

DOI: doi:10.1016/j.cbpc.2004.10.007


Digestive enzyme activities of three talitrid amphipods were examined to investigate the relationship between their digestive capabilities and diet. Laminarinase, cellobiase, carboxymethyl-cellulase, xylanase, α- and β-glucosidase and lipase were detected in all three species suggesting talitrid amphipods can readily digest dietary carbohydrate and lipid, including complex polysaccharides. Relatively high specific enzyme activity (Units (mg -1 digestive tract protein) -1) of laminarinase and lipase was detected in Talorchestia marmorata, a supralittoral kelp feeder which is coherent with the digestion of lipid-esters and β-glucans (laminarin) which are the main lipid and storage polysaccharides of brown seaweeds. Talorchestia sp., a low shore intertidal feeder, had high enzymatic activity of α- and β-glucosidase, cellobiase and xylanase, which is consistent with the digestion of diatoms. Keratroides vulgaris, a forest litter feeder had a relatively low specific activity of all enzymes. It is possible that leaf litter is partially digested prior to ingestion by bacteria and fungi present in the rotting vegetation, with bacterial and fungal enzymes contributing to this species' ability to hydrolyse its diet. This study provides the first quantitative data on digestive capacity in these three talitrid amphipods and confirms the relationship between dietary preference and digestive enzyme complement. © 2004 Elsevier Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Enzymes
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Johnston, MD (Mr Matthew Johnston)
UTAS Author:Johnston, DJ (Dr Danielle Johnston)
UTAS Author:Richardson, AMM (Associate Professor Alastair Richardson)
ID Code:32735
Year Published:2005
Web of Science® Times Cited:27
Deposited By:TAFI - Zoology
Deposited On:2005-08-01
Last Modified:2011-09-30

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