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Antarctic distribution, pigment and lipid composition, and molecular identification of the brine dinoflagellate Polarella glacialis (Dinophyceae)

Citation

Thomson, P and Wright, SW and Bolch, CJS and Nichols, PD and Skerratt, J and McMinn, A, Antarctic distribution, pigment and lipid composition, and molecular identification of the brine dinoflagellate Polarella glacialis (Dinophyceae), Journal of Phycology, 40, (5) pp. 867-873. ISSN 0022-3646 (2004) [Refereed Article]

DOI: doi:10.1111/j.1529-8817.2004.03169.x

Abstract

Polarella glacialis (Montresor et al.) was identified in Davis Station sea ice by morphological and DNA sequence comparison of cultures with those of the authentic strain P. glacialis CCMP 1383 isolated from McMurdo Sound. Cells and cysts of the Davis isolate (FL1B) were morphologically indistinguishable from P. glacialis, and comparison of the large subunit rDNA of both cultures demonstrated only 0.2% sequence divergence over 1366 base pairs. The photosynthetic pigments of P. glacialis (strains FL1B and CCMP 1383) were typical of dinoflagellates, with peridinin (contributing up to 31%) as the major accessory pigment. Extremely high levels of polyunsaturated fatty acids (PUFA, up to 76.3%) were characteristic of P. glacialis isolate FL1B. The high PUFA concentration of this species is thought to be an adaptation to survive the cold temperatures of the upper fast ice. The sterol profile of FL1B was atypical of dinoflagellates, with 4-desmethylsterols (up to 79%) in greater abundance than 4α-methyl sterols (up to 24%). 27-Nor-24-methylcholest-5,22E-dien-3β- ol was identified as the principle sterol in P. glacialis, contributing up to 64% of the total sterol composition.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Environment
Objective Group:Natural Hazards
Objective Field:Natural Hazards in Marine Environments
Author:Bolch, CJS (Dr Christopher Bolch)
Author:Nichols, PD (Dr Peter Nichols)
Author:Skerratt, J (Dr Jenny Skerratt)
Author:McMinn, A (Professor Andrew McMinn)
ID Code:32289
Year Published:2004
Web of Science® Times Cited:32
Deposited By:IASOS
Deposited On:2004-08-01
Last Modified:2005-07-06
Downloads:0

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