eCite Digital Repository

Proteomic Analysis of Selected Prognostic Factors of Breast Cancer

Citation

Roberts, K and Bhatia, K and Stanton, P and Lord, RJ, Proteomic Analysis of Selected Prognostic Factors of Breast Cancer, Proteomics, 4, (3) pp. 784-792. ISSN 1615-9853 (2004) [Refereed Article]

DOI: doi:10.1002/pmic.200300633

Abstract

The incidence of breast cancer is on the rise but as yet there is no guaranteed beneficial treatment for many of the sufferers. The treatments specific for breast and other hormone-sensitive cancers work well at times, however, the population of women that they will benefit is relatively small. Many are limited to surgical, chemotherapy, and radiotherapy options. Here, using two-dimensional electrophoresis (2-DE) in conjunction with a silver stain and Western blotting approach, we attempt to locate selected known prognostic markers for breast cancer. With these results, we can exclude these proteins from the future search for potential pharmaceutical targets, using the same techniques. The proteins that were located include the estrogen receptor-α, β-casein, cytokeratin 7, calponin and bax. For each protein an estimated M r and p/was gained. Each protein was found in multiple variants. By locating these proteins the number of unknown proteins found on the 2-DE gel has been reduced, helping the future search for novel markers that are shown as being differentially expressed between healthy and cancerous tissue samples.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Biochemistry and Metabolomics
Research Field:Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Roberts, K (Ms Kylie Roberts)
Author:Bhatia, K (Dr Kiron Bhatia)
Author:Stanton, P (Professor Peter Stanton)
Author:Lord, RJ (Dr Roger Lord)
ID Code:32046
Year Published:2004
Web of Science® Times Cited:14
Deposited By:Surgery
Deposited On:2004-08-01
Last Modified:2005-05-11
Downloads:0

Repository Staff Only: item control page