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Selectivity control in the separation of aromatic amino acid enantiomers with sulphated β-cyclodextrin


Zakaria, P and Macka, M and Haddad, PR, Selectivity control in the separation of aromatic amino acid enantiomers with sulphated β-cyclodextrin, Journal of Chromatography A, 1031, (1-2) pp. 179-186. ISSN 0021-9673 (2004) [Refereed Article]

DOI: doi:10.1016/j.chroma.2003.11.013


Control of selectivity in the enantiomeric separation of three aromatic amino acids (phenylalanine, tyrosine and tryptophan) is demonstrated by electrokinetic capillary chromatography utilising temperature variations coupled with the use of sulphated-β-cyclodextrin (s-β-CD) as a pseudostationary phase. The concentration of s-β-CD and temperature were used as experimental variables to control the observed selectivity. A double-coated capillary was used and proved very robust with reproducibility of migration times being <2.0% R.S.D. between runs and <2.6% on using a new capillary. The system was modelled successfully using an artificial neural network (ANN) comprising one input layer, two hidden layers and one output layer. The model accurately described the observed separations with a correlation coefficient of 0.999 being observed between predicted and observed migration times. Selectivity optimisation was achieved using the normalised resolution product and minimum resolution criteria, with both providing optima at different experimental conditions. The selectivity changes observed also allowed the estimation of electrolyte temperatures within the capillary at high operating currents (>100μA). Using a 50μm i.d. capillary and an electrolyte comprising 20mM phosphate and 15mM s-β-CD, a temperature of 52°C was calculated within the capillary at an applied voltage of +30kV. © 2003 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Zakaria, P (Dr Philip Zakaria)
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:31927
Year Published:2004
Web of Science® Times Cited:8
Deposited By:Chemistry
Deposited On:2004-08-01
Last Modified:2011-07-21

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