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Insulin sensitivity of muscle capillary recruitment in vivo

Citation

Zhang, L and Vincent, MA and Richards, SM and Clerk, LH and Rattigan, S and Clark, MG and Barrett, EJ, Insulin sensitivity of muscle capillary recruitment in vivo, Diabetes, 53, (2) pp. 447-453. ISSN 0012-1797 (2004) [Refereed Article]

DOI: doi:10.2337/diabetes.53.2.447

Abstract

We have reported that insulin exerts two vascular actions in muscle; it both increases blood flow and recruits capillaries. In parallel hyperinsulinemic-euglycemic clamp studies, we compared the insulin dose response of muscle microvascular recruitment and femoral blood flow as well as hindleg glucose uptake in fed, hooded Wistar and fasted Sprague-Dawley rats. Using insulin doses between 0 and 30 mU-1 · min-1 · kg-1, we measured microvascular recruitment at 2 h by 1-methylxanthine (1-MX) metabolism or contrast-enhanced ultrasound (CEU), and muscle glucose uptake was measured by either arteriovenous differences or using 2-deoxyglucose. We also examined the time course for reversal of microvascular recruitment following cessation of a 3 mU · min-1 · kg-1 insulin infusion. In both groups, whether measured by 1-MX metabolism or CEU, microvascular recruitment was fully activated by physiologic hyperinsulinemia and occurred at lower insulin concentrations than those that stimulated glucose uptake or hindleg total blood flow. The latter processes were insulin dose dependent throughout the entire dose range studied. Upon stopping the insulin infusion, increases in microvascular volume persisted for 15-30 min after insulin concentrations returned to basal levels. We conclude that the precapillary arterioles that regulate microvascular recruitment are more insulin sensitive than resistance arterioles that regulate total flow.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Diabetes
Author:Zhang, L (Mr Lejun Zhang)
Author:Vincent, MA (Dr Michelle Keske)
Author:Richards, SM (Dr Stephen Richards)
Author:Rattigan, S (Professor Stephen Rattigan)
Author:Clark, MG (Professor Michael Clark)
ID Code:31479
Year Published:2004
Web of Science® Times Cited:112
Deposited By:Biochemistry
Deposited On:2004-08-01
Last Modified:2010-03-24
Downloads:0

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