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Microvascular recruitment is an early insulin effect that regulates skeletal muscle glucose uptake in vivo
Citation
Vincent, MA and Clerk, LH and Lindner, JR and Klibanov, AL and Clark, MG and Rattigan, S and Barrett, EJ, Microvascular recruitment is an early insulin effect that regulates skeletal muscle glucose uptake in vivo, Diabetes, 53, (6) pp. 1418-1423. ISSN 0012-1797 (2004) [Refereed Article]
DOI: doi:10.2337/diabetes.53.6.1418
Abstract
Insulin increases glucose disposal into muscle. In addition, in vivo insulin elicits distinct nitric oxide synthase-dependent vascular responses to increase total skeletal muscle blood flow and to recruit muscle capillaries (by relaxing resistance and terminal arterioles, respectively). In the current study, we compared the temporal sequence of vascular and metabolic responses to a 30-min physiological infusion of insulin (3 mU · min-1 · kg-1, euglycemic clamp) or saline in rat skeletal muscle in vivo. We used contrast-enhanced ultrasound to continuously quantify microvascular volume. Insulin recruited microvasculature within 5-10 min (P < 0.05), and this preceded both activation of insulin-signaling pathways and increases in glucose disposal in muscle, as well as changes in total leg blood flow. Moreover, L-NAME (Nω-nitro-L-arginine-methyl ester), a specific inhibitor of nitric oxide synthase, blocked this early microvascular recruitment (P < 0.05) and at least partially inhibited early increases in muscle glucose uptake (P < 0.05). We conclude that insulin rapidly recruits skeletal muscle capillaries in vivo by a nitric oxide-dependent action, and the increase in capillary recruitment may contribute to the subsequent glucose uptake.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Clinical sciences |
Research Field: | Endocrinology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Vincent, MA (Dr Michelle Keske) |
UTAS Author: | Clark, MG (Professor Michael Clark) |
UTAS Author: | Rattigan, S (Professor Stephen Rattigan) |
ID Code: | 31441 |
Year Published: | 2004 |
Web of Science® Times Cited: | 314 |
Deposited By: | Biochemistry |
Deposited On: | 2004-08-01 |
Last Modified: | 2010-03-24 |
Downloads: | 0 |
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