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Targeted electroporation in Xenopus tadpoles in vivo - from single cells to the entire brain

Citation

Haas, K and Jensen, K and Sin, WC and Foa, LC and Cline, HT, Targeted electroporation in Xenopus tadpoles in vivo - from single cells to the entire brain, Differentiation, 70, (4-5) pp. 148-154. ISSN 0301-4681 (2002) [Refereed Article]

DOI: doi:10.1046/j.1432-0436.2002.700404.x

Abstract

Electroporation is becoming more popular as a technique for transfecting neurons within intact tissues. One of the advantages of electroporation over other transfection techniques is the ability to precisely target an area for transfection. Here we highlight this advantage by describing methods to restrict transfection to either a single cell, clusters of cells, or to include large portions of the brain of the intact Xenopus tadpole. Electroporation is also an effective means of gene delivery in the retina. We have developed these techniques to examine the effects of regulated gene expression on various neuronal properties, including structural plasticity and synaptic transmission. Restriction of transfection to individual cells aids in imaging of neuronal morphology, while bulk cell transfection allows examination of the affects of gene expression on populations of cells by biochemical assays, imaging, and electrophysiological recording.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Foa, LC (Professor Lisa Foa)
ID Code:31360
Year Published:2002
Web of Science® Times Cited:90
Deposited By:Anatomy and Physiology
Deposited On:2005-03-08
Last Modified:2011-08-02
Downloads:0

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