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In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells


Butler, R and Nowak, BF, In vitro interactions between Neoparamoeba sp and Atlantic salmon epithelial cells, Journal of Fish Diseases, 27, (6) pp. 343-349. ISSN 0140-7775 (2004) [Refereed Article]

DOI: doi:10.1111/j.1365-2761.2004.00550.x


Neoparamoeba sp., including the putative aetiological agent of amoebic gill disease in cultured fish (N. pemaquidensis), were incubated in vitro with an Atlantic salmon gill epithelium (RGE-2) cell line. Proliferation by the amoeba population was dependent upon culture osmolarity; no growth occurred at 330 mM kg-1 but a sixfold increase was observed at 1000 mM kg-1. At 780 mM kg-1 there was a fourfold increase in the amoeba population but a concurrent decrease in RGE-2 cell density that was significantly greater than that caused by the high culture osmolarity alone. This apparent cytopathic effect (CPE) developed rapidly and resulted in complete cytolysis of the monolayer in 5 days. CPE occurred in multiple foci and presented as cell vacuolation, rounding and clumping, and the rapid clearance of large areas of the cell monolayer. The possibility that CPE is because of the presence of Neoparamoeba sp. derived cytolytic products is discussed in the context of the pathology of the disease in vivo and the occurrence of secreted cytopathogenic compounds in other amoeba species.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Butler, R (Dr Rick Butler)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
ID Code:31209
Year Published:2004
Web of Science® Times Cited:12
Deposited By:TAFI - Aquaculture
Deposited On:2004-08-01
Last Modified:2005-05-02

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