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BAL eotaxin and IL-5 in asthma, and the effects of inhaled corticosteroid and 2 agonist

Citation

Feltis, BN and Reid, DW and Ward, C and Walters, EH, BAL eotaxin and IL-5 in asthma, and the effects of inhaled corticosteroid and s2 agonist, Respirology, 9, (4) pp. 507-513. ISSN 1323-7799 (2004) [Refereed Article]

DOI: doi:10.1111/j.1440-1843.2004.00624.x

Abstract

Objective: In a longitudinal ex vivo placebo-controlled study, asthmatics already treated with inhaled corticosteroid received supplemental long-acting β-agonist (LABA) or increased doses of their inhaled corticosteroid (ICS). Previously reports have shown significant reductions in biopsy eosinophil numbers after treatment with LABA. Following these findings, eosinophil chemokines eotaxin and IL-5 in the BAL fluid at baseline and after 3 months of study medication have now been measured, and these data with that from new cross-sectional controls have also been compared. It is hypothesised that changes in airway eosinophils would be related to eosinophil cytokines. Methodology: BAL cytokines were measured by chemiluminescent enzyme-linked immunosorbent assay, while eosinophils were measured by immunohistochemistry or differential cell counting. For all measures, longitudinal data were compared to that from ICS-free asthmatics (n = 42) and non-asthmatic controls (n = 28). Results: BAL eotaxin in asthmatics was elevated above non-asthmatic levels regardless of ICS levels. BAL IL-5 was elevated in ICS-free asthmatics, but not in asthmatics on low-dose ICS treatment. Longitudinally, BAL eotaxin was unchanged after 3 months. Unexpectedly, IL-5 increased after 3 months of additional LABA treatment but was not further affected by increasing the dose of ICS. Airway eotaxin seemed to be constitutively raised in asthmatics, whereas, IL-5 levels were more steroid-responsive. No relationship was observed between eosinophils and eosinophilic cytokines in the BAL. Conclusions: While the elevation of luminal IL-5 with LABA treatment cannot be accounted for, it may have contributed to luminal clearance of airway wall eosinophils. The lack of correlation between airways eosinophils and eosinophilic cytokines in the BAL is particularly important.

Item Details

Item Type:Refereed Article
Research Division:Earth Sciences
Research Group:Atmospheric Sciences
Research Field:Atmospheric Sciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Physical Sciences
Author:Feltis, BN (Dr Bryce Feltis)
Author:Reid, DW (Dr David Reid)
Author:Walters, EH (Professor Haydn Walters)
ID Code:30780
Year Published:2004
Web of Science® Times Cited:13
Deposited By:Medicine (Discipline)
Deposited On:2004-08-01
Last Modified:2010-06-05
Downloads:0

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