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Two modes of the light-induced phytochorome A decline - with and without changes in the porportion of its isoforms (phyA' and phyA'): evidence from fluorescence investigations of mutant phyA-3D pea

Citation

Sineshchekov, VA and Weller, JL, Two modes of the light-induced phytochorome A decline - with and without changes in the porportion of its isoforms (phyA' and phyA'): evidence from fluorescence investigations of mutant phyA-3D pea, Journal of Photochemistry and Photobiology, B: Biology, 75, (3) pp. 127-135. ISSN 1011-1344 (2004) [Refereed Article]

DOI: doi:10.1016/j.jphotobiol.2004.05.010

Abstract

Different modes of the phytochrome function are connected with its polymorphism, the major isoforms being phytochromes A and B (phyA and phyB). In its turn, phyA comprises two native species, phyA′ and phyA″, whose precise nature and functions remain obscure. With the use of in situ fluorescence spectroscopy, we investigated their properties in a mutant of pea, phyA-3D, characterized by exaggerated photoresponses and impaired photodestruction of phyA. The mutation is a substitution of alanine by valine at the position 194 in phyA. The phyA-3DphyB and phyB mutants were also investigated. In dark-grown plants, all the lines had the content and properties of the two phyA species very similar to the wild type. However, a considerably more intense reduction in [phyA] without changes in the phyA′/phyA″ equilibrium was found in far-red grown mutant plants suggesting a hypersensitivity of phyA-3D with regard to its autoregulation. On the contrary, under red illumination, a higher stability of phyA-3D was observed confirming our earlier findings. This allows a conclusion that the A194V substitution in phyA-3D not only impairs its destruction but also enhances its signaling ability, suggesting a role of this locus in modulation of its activity. © 2004 Published by Elsevier B.V.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
Author:Weller, JL (Associate Professor Jim Weller)
ID Code:30668
Year Published:2004
Web of Science® Times Cited:5
Deposited By:Plant Science
Deposited On:2004-08-01
Last Modified:2005-06-08
Downloads:0

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