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Bacterial decontamination of on-grown Artemia

Citation

Tolomei, AJ and Burke, CM and Crear, BJ and Carson, J, Bacterial decontamination of on-grown Artemia, Aquaculture, 232, (1-4) pp. 357-371. ISSN 0044-8486 (2004) [Refereed Article]

DOI: doi:10.1016/S0044-8486(03)00540-4

Abstract

The bacterial load of on-grown Artemia was manipulated using a variety of commercially available enrichment DHA boosters, selected algal species (Skeletonema costatum; Nannochloropsis oculata; Tetraselmis suecica; Chaetoceros muelleri), and ozone to decontaminate enteric and external surfaces, respectively. Enrichment in C. muelleri over a 6-h period, with an additional algal exchange mid-enrichment, provided the most efficient method for enteric decontamination as measured by total viable counts. Direct exposure to ozone at 4 ppm for 5 min provided further bacterial reduction, resulting in a combined bacterial load reduction of 99.5% without compromising Artemia viability. The commercial enrichment A1 DHA Selco™, containing antibacterial compounds, provides an alternative to algal-based enrichments, however, its decontaminating properties were inferior. Fluorescence in situ hybridisation (FISH) was used on occasions to verify total bacterial abundance estimates obtained by standard plating procedures. In all except one case, Johnson's Marine Agar (JMA) provided results comparable to direct counts by FISH. This indicates that the bacterial community present in on-grown Artemia cultures is dominated by several fast-growing r-strategists amenable to culture on conventional plates. Underestimation of bacterial abundance using marine agar was therefore unlikely. © 2003 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Tolomei, AJ (Mr Anthony Tolomei)
Author:Burke, CM (Dr Chris Burke)
Author:Crear, BJ (Mr Bradley Crear)
Author:Carson, J (Dr Jeremy Carson)
ID Code:30081
Year Published:2004
Web of Science® Times Cited:27
Deposited By:TAFI - Aquaculture
Deposited On:2004-08-01
Last Modified:2011-09-20
Downloads:0

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