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Molecular Identification of Uncoupling Proteins 2 and 3 in a Carnivorous Marsupial, the Tasmanian Devil (Sarcophilus harrisii)

Citation

Kabat, AP and Rose, RW and West, AK, Molecular Identification of Uncoupling Proteins 2 and 3 in a Carnivorous Marsupial, the Tasmanian Devil (Sarcophilus harrisii), Physiological and Biochemical Zoology, 77, (1) pp. 109-115. ISSN 1522-2152 (2004) [Refereed Article]

DOI: doi:10.1086/383496

Abstract

This study investigated the expression of uncoupling proteins 2 and 3 (UCP2 and UCP3) in the carnivorous marsupial Sarcophilus harrisii. The current study used molecular techniques to ascertain whether this species expresses UCP2 and/or UCP3. This species increases nonshivering thermogenesis in response to cold exposure and norepinephrine, although our previous study was unable to demonstrate the presence of brown adipose tissue or uncoupling protein 1. Samples of skeletal muscle and white adipose tissues were taken from five S. harrisii pre- and post-cold acclimation (2°-3°C for 2 wk). The tissues were examined for UCP2 and UCP3 expression through Western blots and reverse transcriptase polymerase chain reaction, with subsequent sequencing to ensure identification of the desired gene. These data suggest that S. harrisii expresses UCP2 but not UCP3. The sequencing of the amplified S. harrisii UCP2 cDNA has revealed a 76% homology with human UCP2 cDNA and a 72% homology with rat UCP2 cDNA. The expression of UCP2 but not UCP3 suggests that UCP2 is conserved from a common ancestor to both the Marsupialia and the Eutheria taxa.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Physiology
Research Field:Animal Physiology - Cell
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Kabat, AP (Mr Alexander Kabat)
Author:Rose, RW (Associate Professor Randy Rose)
Author:West, AK (Professor Adrian West)
ID Code:29874
Year Published:2004
Web of Science® Times Cited:5
Deposited By:Zoology
Deposited On:2004-08-01
Last Modified:2005-05-21
Downloads:0

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