Effects of dbcAMP on steroidogenesis in isolated ovarian follicles of Atlantic salmon

In vitro estradiol-17β (E2) and testosterone (T) production were measured in vitellogenic follicles of Atlantic salmon in response to treatment with human chorionic gonadotropin (hCG) and 2-0-dibutyryladenosine 3′,5′-cyclic monophosphate (dbcAMP). DbcAMP stimulated T production but had no effect on E2, whereas hCG treatment stimulated E2 production but T production was low. Elevated intracellular [cAMP] may be part of the normal mechanism for inhibition of aromatase activity at the end of vitellogenesis. © 2004 Kluwer Academic Publishers.