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Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces

Citation

Kirov, SM and Castrisios, M and Shaw, JG, Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces, Infection and Immunity, 72, (4) pp. 1939-1945. ISSN 0019-9567 (2004) [Refereed Article]

DOI: doi:10.1128/IAI.72.4.1939-1945.2004

Abstract

Aeromonas spp. (gram-negative, aquatic bacteria which include enteropathogenic strains) have two distinct flagellar systems, namely a polar flagellum for swimming in liquid and multiple lateral flagella for swarming over surfaces. Only ∼60% of mesophilic strains can produce lateral flagella. To evaluate flagellar contributions to Aeromonas intestinal colonization, we compared polar and lateral flagellar mutant strains of a diarrheal isolate of Aeromonas caviae for the ability to adhere to the intestinal cell lines Henle 407 and Caco-2, which have the characteristic features of human intestinal enterocytes. Strains lacking polar flagella were virtually nonadherent to these cell lines, while loss of the lateral flagellum decreased adherence by ∼60% in comparison to the wild-type level. Motility mutants (unable to swim or swarm in agar assays) had adhesion levels of ∼50% of wild-type values, irrespective of their flagellar expression. Flagellar mutant strains were also evaluated for the ability to form biofilms in a borosilicate glass tube model which was optimized for Aeromonas spp. (broth inoculum, with a 16- to 20-h incubation at 37°C). All flagellar mutants showed a decreased ability to form biofilms (at least 30% lower than the wild type). For the chemotactic motility mutant cheA, biofilm formation decreased >80% from the wild-type level. The complementation of flagellar phenotypes (polar flagellar mutants) restored biofilms to wild-type levels. We concluded that both flagellar types are enterocyte adhesins and need to be fully functional for optimal biofilm formation.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Kirov, SM (Associate Professor Sylvia Kirov)
Author:Castrisios, M (Ms Marika Castrisios)
ID Code:29710
Year Published:2004
Web of Science® Times Cited:103
Deposited By:Pathology
Deposited On:2004-08-01
Last Modified:2005-08-12
Downloads:0

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