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Maternal Inheritance of Mitochondria in Eucalyptus globulus


Vaillancourt, RE and Petty, A and McKinnon, GE, Maternal Inheritance of Mitochondria in Eucalyptus globulus, Journal of Heredity, 95, (4) pp. 353-355. ISSN 0022-1503 (2004) [Refereed Article]

DOI: doi:10.1093/jhered/esh051


It is important to verify mitochondrial inheritance in plant species in which mitochondrial DNA (mtDNA) will be used as a source of molecular markers. We used a polymerase chain reaction (PCR)/restriction fragment length polymorphism (RFLP) approach to amplify mitochondrial introns from subunits 1, 4, 5, and 7 of NADH dehydrogenase (nad) and cytochrome oxidase subunit II (cox2) in Eucalyptus globulus. PCR fragments were then either sequenced or cut with restriction enzymes to reveal polymorphism. Sequencing cox2 showed that eucalypts lack the intron between exons 1 and 2. One polymorphism was found in intron 2-3 of nad7 following restriction digests with Hph1. Fifty-four F 1 progeny from seven families with parents distinguishable in their mitochondrial nad7 were screened to show that mitochondria were maternally inherited in E. globulus. These results constitute the first report of mitochondrial inheritance in the family Myrtaceae.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genome structure and regulation
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally sustainable plant production
Objective Field:Environmentally sustainable plant production not elsewhere classified
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
UTAS Author:Petty, A (Mr Anthony Petty)
UTAS Author:McKinnon, GE (Dr Gay McKinnon)
ID Code:29536
Year Published:2004
Web of Science® Times Cited:9
Deposited By:Plant Science
Deposited On:2004-08-01
Last Modified:2009-09-10

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