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Auxin from the Developing Inflorescence Is Required for the Biosynthesis of Active Gibberellins in Barley Stems

Citation

Wolbang, CM and Chandler, PM and Smith, JJ and Ross, JJ, Auxin from the Developing Inflorescence Is Required for the Biosynthesis of Active Gibberellins in Barley Stems, Plant Physiology, 134, (2) pp. 769-776. ISSN 0032-0889 (2004) [Refereed Article]

DOI: doi:10.1104/pp.103.030460

Abstract

Multiple gibberellins (GAs) were quantified in the stems of intact, decapitated, and decapitated auxin-treated barley (Hordeum vulgare) plants. Removal of the developing inflorescence reduced the endogenous levels of indole-3-acetic acid (IAA), GA1, and GA3 and increased the level of GA29 in internodal and nodal tissues below the site of excision. Application of IAA to the excised stump restored GA levels to normal in almost all cases. The conversion of [14C]GA20 to bioactive [14C]GA1 and of [14C]GA5 to bioactive [14C]GA3 was reduced by decapitation, and IAA application was able to restore conversion rates back to the levels found in intact plants. The amount of mRNA for the principal vegetative 3-oxidase (converting GA20 to GA1, and GA5 to GA 3) was decreased in decapitated plants and restored by IAA application. The results indicate that the inflorescence of barley is a source of IAA that is transported basipetally into the internodes and nodes where bioactive GA1 and GA3 are biosynthesized. Thus, IAA is required for normal GA biosynthesis in stems, acting at multiple steps in the latter part of the pathway.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
Author:Wolbang, CM (Miss Carla Wolbang)
Author:Smith, JJ (Ms Jenny Smith)
Author:Ross, JJ (Associate Professor John Ross)
ID Code:29397
Year Published:2004
Web of Science® Times Cited:63
Deposited By:Plant Science
Deposited On:2004-08-01
Last Modified:2007-11-02
Downloads:0

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