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Snapper (Pagrus auratus) leucocyte proliferation is synergistically enhanced by simultaneous stimulation with LPS and PHA


Morrison, RN and Lyons, AB and Nowak, BF and Hayball, JD, Snapper (Pagrus auratus) leucocyte proliferation is synergistically enhanced by simultaneous stimulation with LPS and PHA, Fish & Shellfish Immunology, 16, (3) pp. 307-319. ISSN 1050-4648 (2004) [Refereed Article]

DOI: doi:10.1016/S1050-4648(03)00112-8


Important channels of communication between mammalian leucocytes have long been recognised. Here, data are reported that suggest similar integrations may occur between snapper leucocytes upon mitogen stimulation. Cell surface immunoglobulin (IgM) expression was used in conjunction with intracellular fluorescence staining and flow cytometry to differentiate proliferating peripheral blood leucocyte subsets (PBLs). Independent activation using phytohaemagglutinin (PHA) or lipopolysaccharide (LPS) drove both mIgand mIg+cells into cycle. It is not known if the proliferation of mIg+cells was mediated by a mutually exclusive effect of the mitogen on each cell population, cognate cellular interaction or a soluble growth factor. Simultaneous activation of PBLs with PHA and LPS consistently induced significantly more cells to proliferate than the sum of proliferating cells stimulated solely with PHA or LPS. Together, the results suggest that different leucocyte subsets have the capability to influence their respective responses to mitogenic stimulation. Therefore, like in the mammalian immune system, communication may occur between snapper leucocyte subsets.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Morrison, RN (Dr Richard Morrison)
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
ID Code:29290
Year Published:2004
Web of Science® Times Cited:11
Deposited By:TAFI - Aquaculture
Deposited On:2004-08-01
Last Modified:2018-08-07

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