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LC-MS method for the determination of albuterol enantiomers in human plasma using manual solid-phase extraction and a non-deuterated internal standard

Citation

Jacobson, GA and Chong, FV and Davies, NW, LC-MS method for the determination of albuterol enantiomers in human plasma using manual solid-phase extraction and a non-deuterated internal standard, Journal of Pharmaceutical and Biomedical Analysis, 31, (6) pp. 1237-1243. ISSN 0731-7085 (2003) [Refereed Article]


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DOI: doi:10.1016/S0731-7085(02)00734-3

Abstract

A sensitive enantioselective liquid chromatography–mass spectrometry (LC–MS) assay using a manual solid-phase extraction (SPE) procedure, a non-deuterated internal standard and an ion trap LC–MS was developed to measure (R)- and (S)-albuterol in plasma. Sample extraction from plasma was achieved by a manual SPE extraction procedure with methoxyphenamine added as the internal standard. Chiral separation was achieved using a teicoplanin-based stationary phase and a mobile phase consisting of methanol, acetic acid and 28% (w/v) ammonia (1000:5:1, v/v/v). Samples were analyzed by selected reaction monitoring of product ions from the protonated molecular ions. The detection limit of the assay was 0.1 ng/ml with a conservative lower limit of quantification of 0.25 ng/ml for each enantiomer. Recovery of albuterol enantiomers from plasma spiked at 10 ng/ml of racemate was determined to be 89±5.8% (mean±S.D.). Reproducibility at 10 ng/ml of racemate assessed by the coefficient of variation was found to be 6.5% (n=5). Instrument precision (measured as coefficient of variation) was 1.4% (n=5). The correlation coefficient r2 determined from the calibration curve over the range 0.5–50.0 ng/ml racemate in plasma was 0.998. This assay allows adequate sensitivity, recovery and reproducibility for the application to studies of inhaled albuterol.

Item Details

Item Type:Refereed Article
Keywords: Albuterol; Chiral; Assay; Enantiomer; Liquid chromatography–mass spectrometry
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Clinical Pharmacology and Therapeutics
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Jacobson, GA (Dr Glenn Jacobson)
Author:Chong, FV (Miss Fui Chong)
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:28459
Year Published:2003
Web of Science® Times Cited:27
Deposited By:Pharmacy
Deposited On:2003-08-01
Last Modified:2011-05-27
Downloads:0

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