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Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis

Citation

Ding, C and Li, Q and Xiong, Y and Zhou, AW and Jones, G and Xu, SY, Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis, Clinical and Experimental Immunology, 132, (3) pp. 416-423. ISSN 0009-9104 (2003) [Refereed Article]

DOI: doi:10.1046/j.1365-2249.2003.02167.x

Abstract

The objective of this study was to investigate the effect of the oral administration of type II collagen (CII) on pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis (AA). Sprague-Dawley rats were fed with bovine CII either before immunization with Complete Freund's adjuvant (CFA) or after initiation of arthritis. Hind paw secondary swelling was measured and synoviocytes were harvested. Sera from portal vein of oral tolerized rats were collected and in vitro synoviocytes culture or synoviocytes-Peyer's Patches (PP) cells coculture system were developed. Interleukin (IL)-1 activity was measured by a mouse thymocyte activation assayed by MTT dye reduction and tumour necrosis factor (TNF) activity was measured by an L929 cytotoxicity bioassay. Nitric oxide (NO) and malondialdehyde (MDA) levels were measured by biochemical methods. We found that feeding with CII (5, 50 and 500 μg/kg) for 7 days before immunization significantly suppressed hind paw secondary swelling measured at day 16, 20, 24 and 28 (all P < 0.01) and pro-inflammatory mediator (IL-1, TNF, NO and MDA) production by synoviocytes (all P < 0.01) in rats with AA. Feeding with CII (5, 50 and 500 μg/kg) for 7 days after initiation of arthritis had a similar effect. CII (1, 10, 100 μg/ml) had no effect on IL-1 and TNF production by synoviocytes in vitro, but CII 10 μg/ml suppressed IL-1 and TNF production by synoviocytes-PP cells coculture system (P < 0.01), which was antagonized by anti-TGF-β antibody (10 μg/ml) (P < 0.01). Portal serum (1:10) from oral tolerized rats suppressed IL-1 and TNF production by synoviocytes (P < 0.01), which was also antagonized by anti-TGF-β antibody (10 μg/ml) (P < 0.01).We conclude that oral administration of CII had prophylactic and therapeutic effects on AA and over-production of IL-1,TNF, NO and MDA by synoviocytes was suppressed. Bystander active suppression may be the main mechanism of oral CII in the suppression of synoviocyte function.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Rheumatology and Arthritis
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Skeletal System and Disorders (incl. Arthritis)
Author:Ding, C (Professor Chang-Hai Ding)
Author:Li, Q (Ms Qin Li)
Author:Jones, G (Professor Graeme Jones)
ID Code:28406
Year Published:2003
Web of Science® Times Cited:22
Deposited By:Menzies Centre
Deposited On:2003-08-01
Last Modified:2007-05-29
Downloads:0

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