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Localization of -, -, and -synuclein during neuronal development and alterations associated with the neuronal response to axonal trauma


Quilty, MC and Gai, WP and Pountney, DL and West, AK and Vickers, JC, Localization of a-, a-, and a-synuclein during neuronal development and alterations associated with the neuronal response to axonal trauma, Experimental Neurology, 182, (1) pp. 195-207. ISSN 0014-4886 (2003) [Refereed Article]

DOI: doi:10.1016/S0014-4886(03)00108-0


Genetic and protein studies have indicated abnormalities in α-synuclein in neurodegenerative diseases. However, the developmental localization and cellular role of synuclein isoforms is contentious. We investigated the cellular localization of α-, β-, and γ-synuclein in developing cultured rat neurons and following axonal transection of relatively mature neurons, a model that disrupts the axonal cytoskeleton and results in regenerative sprouting. Cortical neurons were grown up to 21 days in vitro (DIV). Axon bundles at 21 DIV were transected and cellular changes examined at 4 and 24 h post-injury. Immunohistochemistry demonstrated that α- and β-synuclein were localized to cellular cytosol and growth cones at 3DIV, with accumulating puncta-like labeling within axons and growth cones by 10-21DIV. In contrast, γ-synuclein immunoreactivity was limited at all time points. By 21DIV, α- and β-synuclein were present in the same neurons but largely in separate subregions, only 26% of puncta contained both α- and β-synuclein immunoreactivity. Less than 20% of α-, β-, and pan-synuclein immunoreactive puncta directly colocalized to synaptophysin profiles at 10DIV, decreasing to 10% at 21DIV. Both α- and β-synuclein accumulated substantially within damaged axons at 21DIV and were localized to cytoskeletal abnormalities. At latter time points post-injury, α- and β-synuclein immunoreactive puncta were localized to growth cone-like structures in regenerating neurites. This study shows that α- and β-synuclein have a precise localization within cortical neurons and are generally nonoverlapping in their distribution within individual neurons. In addition, synuclein proteins accumulate rapidly in damaged axons and may have a role in regenerative sprouting. © 2003 Elsevier Science (USA). All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell neurochemistry
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Quilty, MC (Miss Marian Quilty)
UTAS Author:West, AK (Professor Adrian West)
UTAS Author:Vickers, JC (Professor James Vickers)
ID Code:28132
Year Published:2003
Web of Science® Times Cited:33
Deposited By:Anatomy and Physiology
Deposited On:2003-08-01
Last Modified:2004-06-16

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