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Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord

Citation

Woodhall, E and West, AK and Vickers, JC and Chuah, MI, Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord, Cellular and Molecular Life Sciences, 60, (10) pp. 2241-2253. ISSN 1420-682X (2003) [Refereed Article]

DOI: doi:10.1007/s00018-003-3265-7

Abstract

Although olfactory ensheathing cells (OECs) are used to promote repair in the injured spinal cord, little is known of their phenotype in this environment. In this study, using quantitative reverse transcriptase-polymerase chain reaction RT-PCR, expression of neuregulin-1 mitogen/survival factors and the axonal growth regulator Nogo was quantified in OECs and compared with other non-neuronal cells. Their expression was also compared with OECs which had previously been encapsulated in a porous polymer tube and implanted into the injured spinal cord. Similar to astrocytes and fibroblasts, OECs expressed various neuregulin subtypes including neu differentiation factor, glial growth factor and sensory and motorneuron-derived factor. Implanted OECs upregulated neu differentiation factor and secreted neuregulin, but downregulated expression of all other variants. OECs and oligodendrocytes expressed Nogo-A, -B and -ABC and were immunopositive for Nogo-A protein. The Nogo-A protein in OECs was found to be cytoplasmic rather than nuclear or cell surface associated. Unlike oligodendrocytes, OECs expressed Nogo-66 receptor (NgR) mRNA. Implanted OECs upregulated Nogo-A and -B, but downregulated Nogo-ABC and NgR.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Neurochemistry
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Woodhall, E (Miss Emma Woodhall)
Author:West, AK (Professor Adrian West)
Author:Vickers, JC (Professor James Vickers)
Author:Chuah, MI (Dr Inn Chuah)
ID Code:28128
Year Published:2003
Web of Science® Times Cited:25
Deposited By:Anatomy and Physiology
Deposited On:2003-08-01
Last Modified:2004-03-22
Downloads:0

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