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Impaired CD40-signalling in Langerhans' cells from murine neonatal draining lymph nodes: implications for neonatally induced cutaneous tolerance

journal contribution
posted on 2023-05-16, 14:37 authored by Simpson, CC, Gregory WoodsGregory Woods, Muller, HK
Cutaneous tolerance to antigens may be induced in mice through application of antigen during the first few days following birth. The mechanism governing this neonatally induced tolerance remains uncertain. We employed a contact hypersensitivity model to analyse dendritic cell (DC) function and the expression of classical and non-classical lymphocyte populations within the neonate. Examination of draining lymph node DC after antigenic challenge of the skin revealed these DC to be significantly deficient in their ability to stimulate antigen-specific T cell proliferation. Co-stimulatory molecule (CD40, CD80 and CD86) expression of these cells was deficient in comparison to adult DC, and functional tests revealed these cells to possess a critical absence of CD40 signalling. A numerical analysis of classical and non-classical lymphocyte expression demonstrated that while the neonatal spleen is devoid of T cells, the lymph nodes have a normal repertoire of T, B, γδ and CD4+CD25+ lymphocytes but an increased expression of natural killer (NK) cells. This study indicates that functionally deficient DC are likely contributors to neonatally induced cutaneous tolerance.

History

Publication title

Clinical and Experimental Immunology

Volume

132

Pagination

201-208

ISSN

0009-9104

Department/School

Tasmanian School of Medicine

Publisher

Blackwell Publishing Ltd

Place of publication

Oxford, UK

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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