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The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase

Citation

Davidson, SE and Elliott, RC and Helliwell, CA and Poole, AT and Reid, JB, The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase, Plant Physiology, 131, (1) pp. 335-344. ISSN 0032-0889 (2003) [Refereed Article]

DOI: doi:10.1104/pp.012963

Abstract

The gibberellin (GA)-deficient dwarf na mutant in pea (Pisum sativum) has severely reduced internode elongation, reduced root growth, and decreased leaflet size. However, the seeds develop normally. Two genes, PsKAO1 and PsKAO2, encoding cytochrome P450 monooxygenases of the subfamily CYP88A were isolated. Both PsKAO1 and PsKAO2 had ent-kaurenoic acid oxidase (KAO) activity, catalyzing the three steps of the GA biosynthetic pathway from ent-kaurenoic acid to GA 12 when expressed in yeast (Saccharomyces cerevisiae). In addition to the intermediates ent-7α-hydroxykaurenoic acid and GA 12-aldehyde, some additional products of the pea KAO activity were detected, including ent-6α,7α-dihydroxykaurenoic acid and 7β-hydroxykaurenolide. The NA gene encodes PsKAO1, because in two independent mutant alleles, na-1 and na-2, PsKAO1 had altered sequences and the five-base deletion in PsKAO1 associated with the na-1 allele cosegregated with the dwarf na phenotype. PsKAO1 was expressed in the stem, apical bud, leaf, pod, and root, organs in which GA levels have previously been shown to be reduced in na plants. PsKAO2 was expressed only in seeds and this may explain the normal seed development and normal GA biosynthesis in seeds of na plants.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Grain Legumes
Author:Davidson, SE (Dr Sandra Davidson)
Author:Elliott, RC (Dr Robert Elliott)
Author:Reid, JB (Professor Jim Reid)
ID Code:27887
Year Published:2003
Web of Science® Times Cited:45
Deposited By:Plant Science
Deposited On:2003-08-01
Last Modified:2012-10-29
Downloads:0

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