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Interference of some matrix ions in cation-exchange chromatography


Divjak, B and Goessler, W and Haddad, PR and Novic, M, Interference of some matrix ions in cation-exchange chromatography, Journal of Chromatography A, 1008, (1) pp. 89-96. ISSN 0021-9673 (2003) [Refereed Article]

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DOI: doi:10.1016 /S0021-9673(03)00976-2


Ion chromatographic analysis of ions in samples containing complex matrix composition strongly depends on the on-column co-processes caused by sample matrix components. In the present publication studies of different separation phenomena in cation-exchange chromatography are described. The studies were performed at ‘non-linear’ chromatographic conditions, when the concentration of matrix (interfering) ions significantly exceeded the concentration of the eluent ions. During the research work, the processes already identified in anion-exchange chromatography, i.e. self-elution, on-column change of the eluent and sample-induced micro-gradient elution were used to explain the chromatographic behavior of alkaline and earth-alkaline cations when samples with high matrix cation concentration were analyzed. When present at higher concentrations, the ammonium cation was found to cause self-elution (NH4+ fraction) as well as on-column eluent neutralization due to its ability to diffuse into/back from the porous core of the stationary phase (NH3 fraction). Co-elution of a matrix component and analytes of interest caused spectroscopic interferences that additionally influenced the peak shape of each individual analyte

Item Details

Item Type:Refereed Article
Keywords:Alkaline metals; Alkaline earth metals; Inorganic cations
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:27355
Year Published:2003
Web of Science® Times Cited:3
Deposited By:Chemistry
Deposited On:2003-08-01
Last Modified:2010-02-05

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