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Ion-exclusion chromatography with conductimetric detection of aliphatic carboxylic acids on a weakly acidic cation-exchange resin by elution with benzoic acid-beta-cyclodextrin
journal contribution
posted on 2023-05-16, 14:22 authored by Tanaka, K, Mori, M, Xu, Q, Halaleh, MIH, Ikedo, M, Taoda, H, Hu, W, Hasebe, K, Fritz, J, Paul HaddadPaul HaddadIn this study, an aqueous solution consisting of benzoic acid with low background conductivity and β-cyclodextrin (β-CD) of hydrophilic nature and the inclusion effect to benzoic acid were used as eluent for the ion-exclusion chromatographic separation of aliphatic carboxylic acids with different pKa values and hydrophobicity on a polymethacrylate-based weakly acidic cation-exchange resin in the H+ form. With increasing concentration of β-cyclodextrin in the eluent, the retention times of the carboxylic acids decreased due to the increased hydrophilicity of the polymethacrylate-based cation-exchange resin surface from the adsorption of OH groups of β-cyclodextrin. Moreover, the eluent background conductivity decreased with increasing concentration of β-cyclodextrin in 1 mM benzoic acid, which could result in higher sensitivity for conductimetric detection. The ion-exclusion chromatographic separation of carboxylic acids with high resolution and sensitivity was accomplished successfully by elution with a 1 mM benzoic acid-10 mM cyclodextrin solution without chemical suppression. © 2003 Elsevier Science B.V. All rights reserved.
History
Publication title
Journal of Chromatography AVolume
997Issue
1-2Pagination
127-132ISSN
0021-9673Department/School
School of Natural SciencesPublisher
Elsevier Science BVPlace of publication
Amsterdam, NetherlandsRepository Status
- Restricted