Creation and characteristics of phosphatidylcholine stationary phases for the chromatographic separation of inorganic anions
Hu, W and Haddad, PR and Tanaka, K and Mori, M and Tekura, K and Hasebe, K and Ohno, M and Kamo, N, Creation and characteristics of phosphatidylcholine stationary phases for the chromatographic separation of inorganic anions, Journal of Chromatography A, 997, (1-2) pp. 237-242. ISSN 0021-9673 (2003) [Refereed Article]
New stationary phases for chromatographic separation of anions, obtained by loading liposomes made from dimyristolyphosphatidylcholine (DMPC) onto reversed-phase packed columns (C18 and C30) are reported. Mono- and divalent anions were used as model analyte ions and retention data for these species were obtained using the DMPC stationary phases and used to elucidate the separation mechanisms involved in this chromatographic system. The DMPC stationary phases can separate anions by either a solvation-dependent mechanism or an electrostatic ion-exchange mechanism, depending upon the relative magnitudes of the negative electrostatic potential (Ψ(−)) of the phosphate moiety (P−) and the positive electrostatic potential (Ψ(+)) of the quaternary ammonium groups (N+) on the headgroup of DMPC. If Ψ(+)>Ψ(−), such as in case where Ψ(−) has been reduced either by binding of eluent cations (e.g., H+ or divalent cations) onto the P− group of DMPC or by steric screening when a C30 reversed-phase material was used to support the DMPC, then the overall electrostatic surface potential (and hence also the effective anion-exchange capacity) was generally large and the anions were separated on the basis of an electrostatic mechanism. However, if Ψ(+) was similar to Ψ(−), such as in the case of using a C18 reversed-phase support and monovalent cations as eluent cations, then the overall electrostatic surface potential and the effective anion-exchange capacity were very small and the analyte anions were separated on the basis of a solvation-dependent mechanism. The DMPC stationary phases were found to be suitable for the direct determination of iodide and thiocyanate in highly saline water samples, such as seawater samples.