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Degradation of nonane by bacteria from Antarctic marine sediment

Citation

Powell, S and Bowman, JP and Snape, I, Degradation of nonane by bacteria from Antarctic marine sediment, Polar Biology, 27, (10) pp. 573-578. ISSN 0722-4060 (2004) [Refereed Article]

DOI: doi:10.1007/s00300-004-0639-8

Abstract

A microbial enrichment culture tolerant of petroleum hydrocarbons was developed from sediment collected near Casey Station, Antarctica. To select cold-adapted microbes that would degrade diesel, enrichments were cultured at 0°C during six successive transfers to fresh medium, with Special Antarctic Blend diesel (SAB) as the sole carbon source. Biodegradation of components of the SAB was then measured in microcosms inoculated with the enrichment culture. After 16 weeks, the amount of biodegradation was small, but nonane (a C9 alkane) had degraded significantly more in inoculated microcosms than in sterile controls. DNA was then extracted from the enrichment cultures and a fragment of the 16S rRNA gene was amplified for denaturing gradient gel electrophoresis. Bands were excised from the gel and, following sequencing, were found to belong to the genera Pseudomonas and Colwellia. © Springer-Verlag 2004.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Marine Environments
Author:Powell, S (Dr Shane Powell)
Author:Bowman, JP (Associate Professor John Bowman)
ID Code:27112
Year Published:2004
Web of Science® Times Cited:8
Deposited By:Agricultural Science
Deposited On:2006-03-24
Last Modified:2006-03-24
Downloads:0

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