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Detection of the Ability of Tricholoma matsutake to Utilize Sawdust in Aseptic Culture
Citation
Vaario, LM and Gill, WM and Samejima, M and Suzuki, K, Detection of the Ability of Tricholoma matsutake to Utilize Sawdust in Aseptic Culture, Symbiosis, 34, (1) pp. 43-52. ISSN 0334-5114 (2003) [Refereed Article]
Abstract
Four isolates of Tricholoma matsutake were investigated for growth on media supplemented with combinations of glucose and sawdust. After 5 weeks of incubation, the transmission electron microscope observations indicated that hyphae of T. matsutake (T945) hyphae were able to degrade the xylem cell walls of sawdust in a sawdust-containing medium. After 7 weeks of incubation, the quantity of ergosterol produced by three isolates (T945, T61, and Y1) was significantly greater on the medium containing sawdust than the medium without. The activity of p-nitrophenyl-β-D-lactopyranosidase, a cellulolytyic enzyme, was monitored in T. matsutake cultures as a presumptive assay for extracellular cellulolytic activity. p-Nitrophenyl-β-D-lactopyranosidase (p-NPL) activity of T945 was significantly higher on sawdust containing media containing sawdust and 0.1 g/l glucose than on other media. It is suggested that isolate T945 of T. matsutake is capable of utilizing sawdust in aseptic culture. The suitability of beech sawdust as a model substrate in aseptic culture was assessed.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Cellular interactions (incl. adhesion, matrix, cell wall) |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Horticultural crops |
Objective Field: | Horticultural crops not elsewhere classified |
UTAS Author: | Gill, WM (Dr Warwick Gill) |
ID Code: | 27087 |
Year Published: | 2003 |
Web of Science® Times Cited: | 4 |
Deposited By: | Agricultural Science |
Deposited On: | 2003-08-01 |
Last Modified: | 2004-05-03 |
Downloads: | 0 |
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