Four isolates of Tricholoma matsutake were investigated for growth on media supplemented with combinations of glucose and sawdust. After 5 weeks of incubation, the transmission electron microscope observations indicated that hyphae of T. matsutake (T945) hyphae were able to degrade the xylem cell walls of sawdust in a sawdust-containing medium. After 7 weeks of incubation, the quantity of ergosterol produced by three isolates (T945, T61, and Y1) was significantly greater on the medium containing sawdust than the medium without. The activity of p-nitrophenyl-β-D-lactopyranosidase, a cellulolytyic enzyme, was monitored in T. matsutake cultures as a presumptive assay for extracellular cellulolytic activity. p-Nitrophenyl-β-D-lactopyranosidase (p-NPL) activity of T945 was significantly higher on sawdust containing media containing sawdust and 0.1 g/l glucose than on other media. It is suggested that isolate T945 of T. matsutake is capable of utilizing sawdust in aseptic culture. The suitability of beech sawdust as a model substrate in aseptic culture was assessed.

Item Type:	Refereed Article
Research Division:	Biological Sciences
Research Group:	Biochemistry and cell biology
Research Field:	Cellular interactions (incl. adhesion, matrix, cell wall)
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Horticultural crops
Objective Field:	Horticultural crops not elsewhere classified
UTAS Author:	Gill, WM (Dr Warwick Gill)
ID Code:	27087
Year Published:	2003
Web of Science® Times Cited:	4
Deposited By:	Agricultural Science
Deposited On:	2003-08-01
Last Modified:	2004-05-03
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