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Prokaryotic Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments

Citation

Bowman, JP and McCammon, SA and Gibson, JAE and Robertson, LG and Nichols, PD, Prokaryotic Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments, Applied and Environmental Microbiology, 69, (5) pp. 2448-2462. ISSN 0099-2240 (2003) [Refereed Article]

DOI: doi:10.1128/AEM.69.5.2448-2462.2003

Abstract

The prokaryote community activity and structural characteristics within marine sediment sampled across a continental shelf area located off eastern Antarctica (66°S, 143°E; depth range, 709 to 964 m) were studied. Correlations were found between microbial biomass and aminopeptidase and chitinase rates, which were used as proxies for microbial activity. Biomass and activity were maximal within the 0- to 3-cm depth range and declined rapidly with sediment depths below 5 cm. Most-probable-number counting using a dilute carbohydrate-containing medium recovered 1.7 to 3.8% of the sediment total bacterial count, with mostly facultatively anaerobic psychrophiles cultured. The median optimal growth temperature for the sediment isolates was 15°C. Many of the isolates identified belonged to genera characteristic of deep-sea habitats, although most appear to be novel species. Phospholipid fatty acid (PLFA) and isoprenoid glycerol dialkyl glycerol tetraether analyses indicated that the samples contained lipid components typical of marine sediments, with profiles varying little between samples at the same depth; however, significant differences in PLFA profiles were found between depths of 0 to 1 cm and 13 to 15 cm, reflecting the presence of a different microbial community. Denaturing gradient gel electrophoresis (DGGE) analysis of amplified bacterial 16S rRNA genes revealed that between samples and across sediment core depths of 1 to 4 cm, the community structure appeared homogenous; however, principal-component analysis of DGGE patterns revealed that at greater sediment depths, successional shifts in community structure were evident. Sequencing of DGGE bands and rRNA probe hybridization analysis revealed that the major community members belonged to delta proteobacteria, putative sulfide oxidizers of the gamma proteobacteria, Flavobacteria, Planctomycetales, and Archaea. r]RNA hybridization analyses also indicated that these groups were present at similar levels in the top layer across the shelf region.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Antarctic and Sub-Antarctic Flora, Fauna and Biodiversity
Author:Bowman, JP (Associate Professor John Bowman)
Author:McCammon, SA (Ms Sharee McCammon)
Author:Robertson, LG (Ms Lisette Robertson)
ID Code:26999
Year Published:2003
Web of Science® Times Cited:94
Deposited By:Agricultural Science
Deposited On:2003-08-01
Last Modified:2004-05-03
Downloads:0

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