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Exposure to Thiodan« results in lipofuscin accumulation in hepatocytes of the freshwater catfish Tandanus tandanus

Citation

Nowak, BF and Kingsford, MJ, Exposure to Thiodan results in lipofuscin accumulation in hepatocytes of the freshwater catfish Tandanus tandanus, Diseases of Aquatic Organisms, 56, (2) pp. 135-143. ISSN 0177-5103 (2003) [Refereed Article]

DOI: doi:10.3354/dao056135

Abstract

We investigated the effect of time after pulse exposure to 1.0 μg 1 -1 endosulfan (applied as Thiodan®) on endosulfan residues in the liver and ultrastructural changes in the hepatocytes of the freshwater catfish Tandanus tandanus. Time after exposure did not affect the mean residue level in the liver. After exposure to endosulfan, residues in the liver were 227.47 μg kg-1 after 1 d and 282.83 μg kg-1 after 28 d; residues in the bile were 313.97 μg kg-1 after 1 d and 334.53 μg kg-1 after 28 d. At the end of 28 d exposure, lipofuscin was present in up to 69% of hepatocytes of fish containing residues of endosulfan, but absent from control fish. There was a statistically significant increase in the percentage of pyknotic nuclei and altered rough endoplasmic reticulum 28 d after exposure. The mean percentage of cells with altered endoplasmic reticulum ranged from 12.93% (Day 1) to 7.50% (Day 28) for control fish, while for exposed fish it increased from 14.30% (Day 1) to 35.00% (Day 28). The mean percentage of cells with pyknotic nuclei increased from 1.1 to 2.1% in control fish and from 3.8 to 9.6% in exposed fish. Other ultrastructural changes included increased ultrastructural heterogeneity, progressive vacuolation and fractionation of rough endoplasmic reticulum, accumulation of lysosomes and residual bodies, intranuclear inclusions and pseudoinclusions, membrane whorls and myelinated bodies. Protracted senescence was one of the main features of endosulfan toxicity to T. tandanus hepatocytes.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:26935
Year Published:2003
Web of Science® Times Cited:7
Deposited By:TAFI - Aquaculture
Deposited On:2003-08-01
Last Modified:2004-03-29
Downloads:0

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