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Exposure to Thiodan® results in lipofuscin accumulation in hepatocytes of the freshwater catfish Tandanus tandanus

journal contribution
posted on 2023-05-16, 14:13 authored by Barbara NowakBarbara Nowak, Kingsford, MJ
We investigated the effect of time after pulse exposure to 1.0 μg 1 -1 endosulfan (applied as Thiodan®) on endosulfan residues in the liver and ultrastructural changes in the hepatocytes of the freshwater catfish Tandanus tandanus. Time after exposure did not affect the mean residue level in the liver. After exposure to endosulfan, residues in the liver were 227.47 μg kg-1 after 1 d and 282.83 μg kg-1 after 28 d; residues in the bile were 313.97 μg kg-1 after 1 d and 334.53 μg kg-1 after 28 d. At the end of 28 d exposure, lipofuscin was present in up to 69% of hepatocytes of fish containing residues of endosulfan, but absent from control fish. There was a statistically significant increase in the percentage of pyknotic nuclei and altered rough endoplasmic reticulum 28 d after exposure. The mean percentage of cells with altered endoplasmic reticulum ranged from 12.93% (Day 1) to 7.50% (Day 28) for control fish, while for exposed fish it increased from 14.30% (Day 1) to 35.00% (Day 28). The mean percentage of cells with pyknotic nuclei increased from 1.1 to 2.1% in control fish and from 3.8 to 9.6% in exposed fish. Other ultrastructural changes included increased ultrastructural heterogeneity, progressive vacuolation and fractionation of rough endoplasmic reticulum, accumulation of lysosomes and residual bodies, intranuclear inclusions and pseudoinclusions, membrane whorls and myelinated bodies. Protracted senescence was one of the main features of endosulfan toxicity to T. tandanus hepatocytes.

History

Publication title

Diseases of Aquatic Organisms

Volume

56

Pagination

135-143

ISSN

0177-5103

Department/School

Institute for Marine and Antarctic Studies

Publisher

Inter-Research

Place of publication

Germany

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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