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Electrophysiological localization of blue light sensory sites in etiolated dicotyledon seedlings


Babourina, OK and Newman, IA and Shabala, SN, Electrophysiological localization of blue light sensory sites in etiolated dicotyledon seedlings, Plant, Cell and Environment, 26, (9) pp. 1505-1514. ISSN 0140-7791 (2003) [Refereed Article]

DOI: doi:10.1046/j.1365-3040.2003.01072.x


Changes induced by blue light (BL) in ion fluxes and pH in etiolated seedlings of dicotyledons (Cucumis sativus L., Phaseolus lunatus L., Pisum sativum L.) were studied using a non-invasive ion-selective microelectrode technique (MIFE). BL induced significant Ca2+ uptake within the first 5 min after onset in upper parts of the whole cucumber seedling. To locate the sites of the BL perception, studies on excised segments were performed. The data obtained demonstrated that the observed Ca2+ uptake is associated with the very top of the hypocotyl hook of cucumber and butter bean, and with the whole epicotyl of pea, although the response was stronger for the upper parts of the epicotyl. Addition of Gd3+ and La3+ into the bathing solution completely prevented Ca2+ influx, implicating the involvement of Ca2+ channels in the regulation of Ca2+ transport during BL exposure. BL-induced changes in H+ and Ca2+ fluxes were not likely to be linked to the one mechanism, as they were separated spatially and pharmacologically.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Babourina, OK (Dr Olga Babourina)
UTAS Author:Newman, IA (Dr Ian Newman)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:26893
Year Published:2003
Web of Science® Times Cited:5
Deposited By:Agricultural Science
Deposited On:2003-08-01
Last Modified:2004-04-21

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